cobetter

Immunoblotting test is also known as western blot, which is a hybrid technology combining high-resolution gel electrophoresis and immunochemical analysis.

Western blot has the advantages of large analytical capacity, high sensitivity and strong specificity. It is the most common method to detect the characteristics, expression and distribution of proteins, and is widely applicated in:

• Study on Gene Expression at Protein Level
• Antibody Activity Test
• Early Diagnosis of Disease

Advantages

• Smooth and flat surface, not easy to curl
• High mechanical strength, easy to be stripped and reprobed
• Evenly distributed pore size, high protein mobility, clear results
• High sensitivity to ensure successful detection of low abundance protein

Application

• Western Blot

• Adsorption Analysis

• Amino Acid Analysis

• N-terminal Protein Sequencing

• Spot and Slit Printing Detection

• Glycoprotein Coloration

• Lipopolysaccharide Analysis

Performance Comparison of Immunoblotting Membrane

Membrane Material	NC	PVDF
Protein binding capacity μg/cm2	Low	High
Mechanical strength	Fragile	Durable
Chemical compatibility	Limited	Compatible with most organic solvents, acids and weak bases
Whether activation is required	No	Methanol activation required
Detection	Chemiluminescenc Fluorescence detection Conventional dyeing	Chemiluminescence Fluorescence detection Conventional dyeing Coomassie blue staining
Reprocessing	Unsuitable	Suitable
Heat resistance	Poor	Good

Company / Model	M Company	T Company	Cobetter TS2590L	Cobetter TS2590H
Application	Common immunoblotting	Common immunoblotting	Especially suitable for low abundance protein detection	Common immunoblotting
Material	PVDF	PVDF	PVDF	PVDF
Pore Size / μm	0.45	0.45	0.45	0.45
Thickness / μm	127	127	91	115
Test Method	Chemiluminescence Substrate Coloration
Results of 45kDa Proteins
Results of 35kDa Proteins
Results of Proteins less than 30kDa

Pore size	0.2μm
Standard width	270mm (can be changed based on requirement )
Length	Based on the requirement from clients

Model	Pore Size	Length (mm)	Width (mm)
TS2590L	0.45 μm	260	3300	High sensitivity model, suitable for detection of low abundance protein..
TS2590L		297	210
TS2590L		210	148
TS2590L		200	200
TS2590L		150	150
TS2590L		84	70
TS2590H	0.45 μm	260	3300	General model, relatively high sensitivity, suitable for detection of high abundance protein
TS2590H		297	310
TS2590H		210	148
TS2590H		200	200
TS2590H		150	150
TS2590H		84	70